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The Conservatory and Botanical Garden of Geneva's Collection of DNA extracts and silica-dry tissue samples used for genetic and phylogenetic analyses

The BCCM consoritum wants to be a solution partner for providing services of quality in microbial and genetic resources for academia and industry. Its mission is to •acquire, preserve and distribute microbial and genetic resources, •identify and characterize these biological materials, •offer customer-oriented services, •increase the understanding of microorganisms and their function in ecosystems, •foster the application of biological resources. The BCCM consortium gathers 7 complementary microbial culture collections that are coordinated by a central at the Belgian Science Policy.

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Fundação Oswaldo Cruz (Fiocruz) promotes health and social development, generates and disseminates scientific and technological knowledge, be an agent of citizenship. These are the concepts that guide the actions of the Oswaldo Cruz Foundation (Fiocruz), under the Ministry of Health, the most prominent institution of science and technology in health in Latin America. Fiocruz has established its headquarters in the campus located in Manguinhos in the North of Rio de Janeiro state, where are the historic buildings of the former Federal Serum Therapy Institute (Instituto Soroterápico Federal). - as the Moorish Pavilion, the Pavilion of the Clock and Stable. Other six units are in the cities of Rio de Janeiro, Belo Horizonte, Salvador, Recife, Manaus, Brasilia and Curitiba. Besides, there are new units that are being structured, as part of the national expansion project of Fiocruz, relating to policies for decentralization of research and training of human resources, promoted by the Federal Government. The new units of Fiocruz that are in development will be implemented in the states of Mato Grosso do Sul, Ceará, Rondônia and Piauí. Fiocruz has as one of its missions to preserve heritage buildings regarded to its history and its fields of activity. The campuses of the Foundation in Rio de Janeiro, in addition to harbor important architectural specimens of various periods, have high potential urban and landscape. The preservation of this heritage is responsibility of technical staff of the Department of Historical Heritage (DPH) of the Oswaldo Cruz House.

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A comprehensive collection of DNA and tissue predominantly from Scandinavian fauna and flora, but also with substantial contributions from other parts of the world

As an aid for research in molecular phylogenetics the Herbarium maintains a collection of material specifically intended for DNA extraction. Botanists at the Missouri Botanical Garden collect leaf samples, preserve them in silica gel and store them at -20º C. Because the samples are carefully prepared and stored, they are likely to give better yields of higher quality DNA than herbarium material. To date, nearly 11,000 samples have been catalogued and are available for distribution.

The museum’s genetic resource collections began more than 40 years ago as curator-driven research material. Over time, we realized that dispersed collections are vulnerable to equipment failures and sample mismanagement or loss. Therefore, we began an organized effort to centralize these collections, first within some research departments, and then throughout the museum. In 2010 NMNH constructed a biorepository, which began receiving collections in 2011. The current capacity is approximately 4.2 million 2 ml cryovials that are housed in 76 ultra-cold mechanical and liquid nitrogen freezers and a small number of refrigerators. All cold storage units are monitored constantly for performance. NMNH uses a modified version of FreezerPro (Ruro.com) to maintain and track inventory. FreezerPro is connected via an Application Programming Interface (API) to KE EMu, the museum’s catalog system. EMu provides a limited form of the catalog information to allow easier identification of samples. Each vial is assigned a locally unique Biorepository Number by FreezerPro to facilitate a unified system for locating and identifying samples. The collection includes insects, birds, terrestrial plants, marine and terrestrial invertebrates, algae, fish, reptiles, mammals, amphibians, bacteria and protozoans. Current strengths are in bird, marine and terrestrial mammal, and insect holdings, although significant projects have been initiated that will broaden and deepen the taxonomical representation as well as environments. Human tissues, commercial, and agricultural products are not a part of the collection. A complete inventory of all holdings is underway. The entire collection presently numbers more than 250,000 and could be twice that number. All these materials are available for use in genomic research, toxicology studies and environmental monitoring.

The University of Kansas Ichthyology frozen tissue collection continues to expand rapidly and has broad representation of both marine and freshwater fish diversity - 11,000 individual tissue samples from 2384 taxa (297 families and 1077 genera) and 38 countries (Australia, Belize, Ethiopia, Fiji, Nepal, Seychelles, South Africa and Tonga etc., as well as oceanic localities). The collections and the scope of research activities in the division continue to grow due to the ongoing activities of ichthyology staff and students. The collection is used by national and international researchers as well as by state and federal agencies. The Division of Ichthyology is designated as a Regional Center in the Midwest and Great Plains Regions (Collette & Lachner 1976, Copeia 1976: 625-642; Poss and Collette 1995, Copeia 1995: 48-70) and is among the top twenty ichthyological collections in the country. Almost 60% of the specimens in the collection are from the Great Plains Region. The collection is an important resource for anyone interested in the region’s fishes. The data concerning these faunas are not extensively duplicated by other ichthyological collections. The tissue collection comprises tissue samples originally collected in liquid nitrogen, DMSO and ethanol and stored in state-of-the-art liquid nitrogen dewars at -170°C. The tissues are made up mostly of muscle tissue but also includes, liver and other internal organs, fin clips and whole specimens. A large proportion of our collection has vouchers held either at KU or at other collections. The provenance of these vouchers is indicated in the database

The Biobank of the Leibniz Institute for the Analysis of Biodiversity Change (LIB, Bonn and Hamburg) archives samples of animal and environmental DNA, fixed or snap-frozen tissue, and cell cultures, associated with the corresponding biological data. Storage temperatures: -190°C (liquid nitrogen), -80°C, -40°C, -20°C, ambient (sealed). Further info: https://bonn.leibniz-lib.de/en/biobank

The DNA bank of the Botanic Garden and Botanical Museum Berlin-Dahlem holds currently a collection of 20.000 plant DNA and tissue samples growing constantly. Its core collection focuses on the flora of the Berlin and Brandenburg but it also preserves DNA from the Alps, the Mediterranean and the Altai Mountains as well as more than 2000 diatom DNA samples.

Collection of mostly tissues and some DNA from wild vertebrates, with special focus on Central European fauna.

FIOCRUZ/CLIOC, acting as a "Biological Resource Centre" (as defined by the Organisation for Economic Co-operation and Development, OECD), is dedicated to the preservation, storage, distribution, taxonomic characterization, and identification of Leishmania and associated information, thus contributing to the scientific and technological development of Brazil. In addition to developing specific research projects, CLIOC meets the demands of public research and education institutions or industry sectors, providing specialized services.

The Museum of Southwestern Biology (MSB), Division of Genomic Resources (DGR) is a centralized repository at the University of New Mexico (UNM) for cryogenic biological materials submitted from MSB divisions at the University of New Mexico and from other individuals and institutions worldwide. The MSB DGR collection archives cryogenically preserved samples of animal tissues, whole organisms such as embryos and parasites, and purified DNA and RNA for the MSB divisions of Mammals, Birds, Fishes, Herpetology, and Parasitology. The MSB DGR collection contains over 500,000 cryogenic samples of more than 250,000 specimens and 3,000 species, representing Mammals (92%), Birds (4%), Reptiles (1%), Fishes (1%), and a growing collection of associated endo- and ectoparasites (2%). The collection spans more than 30 countries, with particularly strong holdings from the Southwestern United States, Beringia, and Latin America. Tissues and museum specimens can be located online bysearching the Arctos collections database at https://arctosdb.org for each of the MSB divisions.

CBG Collections maintains a globally unique natural history collection of 3.3 million specimens. Every specimen is digitized, and the exact storage location of each specimen is tracked in a collection management information system for quick reference and retrieval. The databased information for every voucher is also archived in the Barcode of Life Data System (BOLD; www.boldsystems.org), permitting the permanent storage, validation and analysis of barcode sequence data and associated specimen metadata. Most (88.6%) of the specimens have been DNA barcoded, and a few representatives of every species have been digitally imaged.

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The Ocean Genome Legacy Center (OGL) is a non-profit marine DNA and tissue repository dedicated to exploring and preserving the wealth of information contained in the genomes of endangered, rare, unusual and ecologically critical marine organisms. OGL’s mission is to collect, describe, and preserve genomes from marine species, and to make these materials widely available for scientific research. By providing secure storage and broad public access to genomic materials and a forum for sharing samples, data, and ideas, OGL aims to serve as a catalyst for research that can help to protect marine ecosystems and improve the human condition. Detailed data are available for each specimen listed in our public online catalog.